预电泳，是除去凝胶中没有聚合的掌维色身死阳英单体和双体和聚合引发剂，提高分辨率的一种做法，不加任何物质，一般30-60分钟后再加样电泳。

• 中文名 预电泳
• 需要时间 20-30min
• 目的 清除凝胶内的杂质
• 做法 将凝胶安置于电泳槽中

做法

　　将聚合好的凝胶安置于电泳槽中，小心拔去梳子，加入电泳缓冲液后低电压10-20V，预电泳20-30min，目的是清除凝胶内的杂质来自， 疏通凝胶孔径以保证电360百科泳过程中电泳的畅通。

　　预电泳后依次加入标准品和待分析样品。(加样时间要尽量短，以免样品扩散，可在未加样的孔中加入等量的样品缓冲液避免边缘效应。样品一般在1.0mm厚费台七获标的胶 加样50-100ug/lane)出用。

　　It is re脚每供必置林木零需图ally depend星多真原牛山最余氢快ent on what gel you are talking about, pro-run for different gels has different purpose. In some case, for instance, SDS PAGE, there may be some residue chemicals in your gel, such as TEMED or ammonium persulfate. Pre-electrophor的见步许背宽怀它esis will move those ahead 优尼展连世of your samples. Some times, people put some chemicals in t江自再也料宽he gel, then the pre-run makes the chemical react with something else. At these day, very few people would do pre-electrophoresis to SDS PAGE, but some other electrophoresis may require the pre-run. I景然剧风剂谓序t really depends.

目的

　　检测是否通电，同时根据相应的化学实验用案达到其他的一些检测和预制实验条件。